产品名称
Claspin (Phospho-Ser30) Rabbit Polyclonal Antibody
蛋白名称
Claspin (Phospho-Ser30)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=63967
Human Swissprot No.
Q9HAW4
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q9HAW4/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/269582
Mouse Swissprot No.
Q80YR7
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q80YR7
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/0
特异性
This antibody detects endogenous levels of Claspin (Phospho-Ser30) at Human, Mouse,Rat
运输及保存条件
-15°C to -25°C/1 year(Do not lower than -25°C)
组织表达
Bone marrow,Epithelium,PCR rescued clones,Skin,
功能
domain:The C-terminus of the protein contains 3 potential CHEK1-binding motifs (CKB motifs). Potential phosphorylation sites within CKB motif 1 and CKB motif 2 are required for interaction with CHEK1.,function:Required for checkpoint mediated cell cycle arrest in response to inhibition of DNA replication or to DNA damage induced by both ionizing and UV irradiation. Adapter protein which binds to BRCA1 and the checkpoint kinase CHEK1 and facilitates the ATR-dependent phosphorylation of both proteins. Can also bind specifically to branched DNA structures and may associate with S-phase chromatin following formation of the pre-replication complex (pre-RC). This may indicate a role for this protein as a sensor which monitors the integrity of DNA replication forks.,induction:Expression peaks at S/G2 phases of the cell cycle.,PTM:Phosphorylated during activation of DNA replication or DNA damage checkpoints. This requires ATR.,sequence caution:Contaminating sequence. Potential poly-A sequence.,similarity:Belongs to the claspin family.,subunit:Interacts with CHEK1 when phosphorylated. Interacts with ATR and RAD9A and these interactions are slightly reduced during checkpoint activation. Interacts with BRCA1 and this interaction increases during checkpoint activation. Interacts with TIMELESS.,
纯化
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
