产品名称
CD150 (Phospho-Tyr327) Rabbit Polyclonal Antibody
别名
Signaling lymphocytic activation molecule (CDw150) (IPO-3) (CD antigen CD150)
蛋白名称
CD150 (Phospho-Tyr327)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=6504
Human Swissprot No.
Q13291
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q13291/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/27218
Mouse Swissprot No.
Q9QUM4
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q9QUM4
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/0
特异性
This antibody detects endogenous levels of CD150 (Phospho-Tyr327) at Human, Mouse,Rat
运输及保存条件
-15°C to -25°C/1 year(Do not lower than -25°C)
组织表达
Constitutively expressed on peripheral blood memory T-cells, T-cell clones, immature thymocytes and a proportion of B-cells, and is rapidly induced on naive T-cells after activation (PubMed:7617038). Activated B-cells express isoform 1, isoform 3 and a cytoplasmic isoform (PubMed:9091591). Isoform 4 is expressed in B-cells, primary T-cells, dendritic cells and macrophages. Isoform 4 is expressed in tumors of the central nervous system (PubMed:25710480).
细胞定位
Cell membrane ; Single-pass type I membrane protein. Present on the surface of B-cells and T-cells. Located at the plasma membrane contacts between neighboring T-cells (PubMed:11806999). .; [Isoform 3]: Secreted .; [Isoform 4]: Cell membrane . Overexpressed isoform 4 is detected on the cell surface. In glioma cell lines endogenuous isoform 4 is detetced predominantly in the cytoplasm and colocalized with endoplasmic reticulum and Golgi markers. .
功能
domain:The most membrane-proximal SH2-binding motif interacts with SH2 domain of SH2D1A and does not need to be phosphorylated on tyrosine residues.,function:High-affinity self-ligand important in bidirectional T-cell to B-cell stimulation. SLAM-induced signal-transduction events in T-lymphocytes are different from those in B-cells. Two modes of SLAM signaling are likely to exist: one in which the inhibitor SH2D1A acts as a negative regulator and another in which protein-tyrosine phosphatase 2C (PTPN11)-dependent signal transduction operates.,PTM:Phosphorylated by FYN.,similarity:Contains 1 Ig-like C2-type (immunoglobulin-like) domain.,similarity:Contains 1 Ig-like V-type (immunoglobulin-like) domain.,subcellular location:Present on the surface of B-cells and T-cells.,subunit:Its cytoplasmic domain interacts with SH2 domain protein 1A (SH2D1A), and with PTPN11. Interacts with INPP5D/SHIP1. Binds to Measles virus HN protein and acts as a receptor for this virus.,tissue specificity:Constitutively expressed on peripheral blood memory T-cells, T-cell clones, immature thymocytes, and a proportion of B-cells, and is rapidely induced on naive T-cells after activation.,
纯化
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
