产品名称
Ack1 (Phospho-Tyr857+Tyr858) Rabbit Polyclonal Antibody
别名
Activated CDC42 kinase 1 (ACK-1) (EC 2.7.10.2) (EC 2.7.11.1) (Tyrosine kinase non-receptor protein 2)
蛋白名称
Ack1 (Phospho-Tyr857+Tyr858)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=10188
Human Swissprot No.
Q07912
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q07912/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/51789
Mouse Swissprot No.
O54967
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/O54967
Rat Gene Link
https://www.uniprot.org/uniprot/303882
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/Q5U2X5
特异性
This antibody detects endogenous levels of Ack1 (Phospho-Tyr857+Tyr858) at Human, Mouse,Rat
运输及保存条件
-15°C to -25°C/1 year(Do not lower than -25°C)
组织表达
The Tyr-284 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages. It also shows a significant increase in expression in prostate cancers during the progressive stages.
细胞定位
Cell membrane . Nucleus . Endosome . Cell junction, adherens junction . Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side . Cytoplasmic vesicle, clathrin-coated vesicle . Membrane, clathrin-coated pit . Cytoplasm, perinuclear region . Cytoplasm, cytosol . The Tyr-284 phosphorylated form is found both in the membrane and nucleus (By similarity). Co-localizes with EGFR on endosomes (PubMed:20333297). Nuclear translocation is CDC42-dependent (By similarity). Detected in long filamentous cytosolic structures where it co-localizes with CTPS1 (By similarity). .
功能
catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,cofactor:Magnesium.,enzyme regulation:The SH3 domain appears to play an autoinhibitory role.,function:Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR. Participates in clathrin-mediated endocytosis. May be involved both in adult synaptic function and plasticity and in brain development.,sequence caution:Unlikely isoform. Aberrant splice sites.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family.,similarity:Contains 1 CRIB domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SH3 domain.,subunit:Interacts with CDC42. Interacts with activated CSPG4.,
纯化
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
