产品名称
NTHL1 Rabbit Polyclonal Antibody
蛋白名称
Endonuclease III-like protein 1 (EC 4.2.99.18)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
P78549
Human Swissprot Link
https://www.uniprot.org/uniprotkb/P78549/entry
Mouse Swissprot No.
O35980
Mouse Swissprot Link
http://www.uniprot.org/uniprot/O35980
免疫原
Synthesized peptide derived from part region of human protein
特异性
NTHL1 Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene is a DNA N-glycosylase of the endonuclease III family. Like a similar protein in E. coli, the encoded protein has DNA glycosylase activity on DNA substrates containing oxidized pyrimidine residues and has apurinic/apyrimidinic lyase activity. [provided by RefSeq, Oct 2008],
组织表达
Widely expressed with highest levels in heart and lowest levels in lung and liver.
细胞定位
Nucleus . Mitochondrion .
信号通路
Base excision repair;
功能
catalytic activity:The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.,caution:It is uncertain whether Met-1, Met-9 or Met-16 is the initiator.,cofactor:Binds 1 4Fe-4S cluster. The cluster is not important for the catalytic activity, but which is probably involved in the proper positioning of the enzyme along the DNA strand.,developmental stage:Expression levels are regulated during the cell cycle with increased levels during early and mid S-phase.,function:Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA damage and spontaneous mutagenic lesions.,similarity:Belongs to the nth/mutY family.,tissue specificity:Widely expressed with highest levels in heart and lowest levels in lung and liver.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
