产品名称
TOPBP1 (Phospho-Ser1159) Rabbit Polyclonal Antibody
别名
DNA topoisomerase 2-binding protein 1 (DNA topoisomerase II-beta-binding protein 1) (TopBP1) (DNA topoisomerase II-binding protein 1)
蛋白名称
TOPBP1 (Phospho-Ser1159)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=11073
Human Swissprot No.
Q92547
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q92547/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/235559
Mouse Swissprot No.
Q6ZQF0
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q6ZQF0
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/0
特异性
This antibody detects endogenous levels of TOPBP1 (Phospho-Ser1159) at Human, Mouse,Rat
运输及保存条件
-15°C to -25°C/1 year(Do not lower than -25°C)
组织表达
Highly expressed in heart, brain, placenta, lung and kidney.
细胞定位
Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Chromosome. Detected on unpaired autosomes in meiotic prophase cells. Detected on X and Y chromosomes during later stages of prophase. Colocalizes with ATR and H2AX at unsynapsed chromosome cores during prophase (By similarity). Has a uniform nuclear distribution during G phase. Colocalizes with BRCA1 at stalled replication forks during S phase. In mitotic cells it colocalizes with BRCA1 at spindle poles and centrosomes during metaphase and anaphase. Detected in discrete foci together with PML and numerous DNA repair enzymes after DNA damage by alkylating agents, UV or gamma irradiation. Localizes to sites of DNA damage in a H2AX- independent manner. .
功能
function:Required for DNA replication. Plays a role in the rescue of stalled replication forks and checkpoint control. Binds double-stranded DNA breaks and nicks as well as single-stranded DNA. Recruits the SWI/SNF chromatin remodeling complex to E2F1-responsive promoters. Down-regulates E2F1 activity and inhibits E2F1-dependent apoptosis during G1/S transition and after DNA damage. Induces a large increase in the kinase activity of ATR.,induction:Up-regulated during the S phase of the cell cycle. Up-regulated by E2F1 and interferon.,PTM:Phosphorylated on serine and threonine residues in response to X-ray irradiation.,PTM:Ubiquitinated and degraded by the proteasome. X-ray irradiation reduces ubiquitination.,similarity:Contains 8 BRCT domains.,subcellular location:Detected on unpaired autosomes in meiotic prophase cells. Detected on X and Y chromosomes during later stages of prophase. Colocalizes with ATR and H2AFX at unsynapsed chromosome cores during prophase (By similarity). Has a uniform nuclear distribution during G phase. Colocalizes with BRCA1 at stalled replication forks during S phase. In mitotic cells it colocalizes with BRCA1 at spindle poles and centrosomes during metaphase and anaphase. Detected in discrete foci together with PML and numerous DNA repair enzymes after DNA damage by alkylating agents, UV or gamma irradiation.,subunit:Binds E2F1, PML, RAD9A, SMARCA2, SMARCA4, POLE and UBR5. May interact with TOP2B.,tissue specificity:Highly expressed in heart, brain, placenta, lung and kidney.,
纯化
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
