BD-PP1707

TORC2 Phospho-Ser171 Rabbit Polyclonal Antibody

CREB-regulated transcription coactivator 2 (Transducer of regulated cAMP response element-binding protein 2) (TORC-2) (Transducer of CREB protein 2)

常规抗体

CRTC2 TORC2

TORC2 Phospho-Ser171

WB

Human,Mouse,Rat

1 mg/ml

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

200186

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=200186

Q53ET0

https://www.uniprot.org/uniprotkb/Q53ET0/entry

74343

https://www.uniprot.org/uniprot/74343

Q3U182

https://www.uniprot.org/uniprotkb/Q3U182

310615

https://www.uniprot.org/uniprot/310615

Q3LRZ1

https://www.uniprot.org/uniprotkb/Q3LRZ1

This antibody detects endogenous levels of TORC2 Phospho-Ser171 at Human, Mouse,Rat

WB 1:500-2000

76kD

-15°C to -25°C/1 year(Do not lower than -25°C)

Most abundantly expressed in the thymus. Present in both B and T-lymphocytes. Highly expressed in HEK293T cells and in insulinomas. High levels also in spleen, ovary, muscle and lung, with highest levels in muscle. Lower levels found in brain, colon, heart, kidney, prostate, small intestine and stomach. Weak expression in liver and pancreas.

Cytoplasm . Nucleus . Translocated from the nucleus to the cytoplasm on interaction of the phosphorylated form with 14-3-3 protein (PubMed:15454081). In response to cAMP levels and glucagon, relocated to the nucleus (PubMed:15454081). .

function:Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. Regulates the expression of specific genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR).,polymorphism:Variant Cys-379, under a dominant model, linked to a recessive mutation in LKB1, may be asssociated with susceptibility to type II or non-insulin-dependent diabetes mellitus (NIDDM).,PTM:Phosphorylation/dephosphorylation states of Ser-171 are required for regulating transduction of CREB activity. TORCs are inactive when phosphorylated, and active when dephosphorylated at this site. This primary site of phosphorylation, is regulated by cAMP and calcium levels and is dependent on the phosphorylation of SIKs by LKB1. Both insulin and AMPK increase this phosphorylation, of TORC2 while glucagon suppresses it.,similarity:Belongs to the TORC family.,subcellular location:Translocated from the nucleus to the cytoplasm on interaction of the phosphorylated form with 14-3-3 protein. In response to cAMP levels and glucagon, relocated to the nucleus.,subunit:Binds, as a tetramer, through its N-terminal region, with the bZIP domain of CREB1. 'Arg-314' in the bZIP domain of CREB1 is essential for this interaction. Interaction, via its C-terminal, with TAF4, enhances recruitment of TAF4 to CREB1 (By similarity). Interacts with PPP3CA/calcineurin alpha, SNF1LK2 and 14-3-3 proteins, YWHAB and YWHAG. Interaction with the human T-cell leukemia virus type 1 (HTLV-1) Tax protein is essential for optimal transcription activation by Tax. Interaction with RFWD2/COP1 mediates nuclear export and degradation of TORC2.,tissue specificity:Most abundantly expressed in the thymus. Present in both B and T lymphocytes. Highly expressed in HEK293T cells and in insulinomas. High levels also in spleen, ovary, muscle and lung, with highest levels in muscle. Lower levels found in brain, colon, heart, kidney, prostate, small intestine and stomach. Weak expression in liver and pancreas.,

现货

The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.