产品名称
ILK-1 (Phospho-Ser343) Rabbit Polyclonal Antibody
别名
Integrin-linked protein kinase (EC 2.7.11.1) (59 kDa serine/threonine-protein kinase) (ILK-1) (ILK-2) (p59ILK)
蛋白名称
ILK-1 (Phospho-Ser343)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=3611
Human Swissprot No.
Q13418
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q13418/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/16202
Mouse Swissprot No.
O55222
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/O55222
Rat Gene Link
https://www.uniprot.org/uniprot/170922
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/Q99J82
特异性
This antibody detects endogenous levels of ILK-1 (Phospho-Ser343) at Human, Mouse,Rat
运输及保存条件
-15°C to -25°C/1 year(Do not lower than -25°C)
组织表达
Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.
细胞定位
Cell junction, focal adhesion . Cell membrane; Peripheral membrane protein; Cytoplasmic side . Cell projection, lamellipodium . Cytoplasm, myofibril, sarcomere .
功能
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,domain:A PH-like domain is involved in phosphatidylinositol phosphate binding.,enzyme regulation:Stimulated rapidly but transiently by both cell fibronectin interactions, as well as by insulin, in a PI3-K-dependent manner, likely via the binding of PtdIns(3,4,5)P3 with a PH-like domain of ILK.,function:Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.,PTM:Autophosphorylated on serine residues.,similarity:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.,similarity:Contains 1 protein kinase domain.,similarity:Contains 5 ANK repeats.,subunit:Interacts with cytoplasmic domain of beta 1 subunit of integrin. Could also interacts with beta 2, beta 3 and/or beta 5 subunit of integrin. Interacts (via ANK repeats) with LIMS1 and LIMS2. Interacts with parvins and probably TGFB1I1.,tissue specificity:Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.,
纯化
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
