产品名称
CDK5 (Phospho-Ser159) Rabbit Polyclonal Antibody
别名
Cyclin-dependent kinase 5 (EC 2.7.11.22) (Cell division protein kinase 5) (Serine/threonine-protein kinase PSSALRE) (Tau protein kinase II catalytic subunit) (TPKII catalytic subunit)
蛋白名称
CDK5 (Phospho-Ser159)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1020
Human Swissprot No.
Q00535
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q00535/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/12568
Mouse Swissprot No.
P49615
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/P49615
Rat Gene Link
https://www.uniprot.org/uniprot/140908
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/Q03114
特异性
This antibody detects endogenous levels of CDK5 (Phospho-Ser159) at Human, Mouse,Rat
运输及保存条件
-15°C to -25°C/1 year(Do not lower than -25°C)
组织表达
[Isoform 1]: Ubiquitously expressed (PubMed:17009320, PubMed:19693690). Accumulates in cortical neurons (at protein level) (PubMed:17009320). ; [Isoform 2]: Expressed in the testis, skeletal muscle, colon, bone marrow and ovary.
细胞定位
[Isoform 1]: Cytoplasm . Nucleus . Cell membrane ; Peripheral membrane protein. Perikaryon. Cell projection, lamellipodium . Cell projection, growth cone . Cell junction, synapse, postsynaptic density . Cell junction, synapse . In axonal growth cone with extension to the peripheral lamellipodia (By similarity). Under neurotoxic stress and neuronal injury conditions, CDK5R (p35) is cleaved by calpain to generate CDK5R1 (p25) in response to increased intracellular calcium. The elevated level of p25, when in complex with CDK5, leads to its subcellular misallocation as well as its hyperactivation. Colocalizes with CTNND2 in the cell body of neuronal cells, and with CTNNB1 in the cell-cell contacts and plasma membrane of undifferentiated and differentiated neuroblastoma cells. Reversibly attached to the plasma membrane in an inactive form when complexed to dephosphorylated p35 or CDK5R2 (p39), p35 phosphorylation releases this attachment and activates CDK5. .; [Isoform 2]: Nucleus.
功能
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Probably involved in the control of the cell cycle. Interacts with D1 and D3-type G1 cyclins. Can phosphorylate histone H1, tau, MAP2 and NF-H and NF-M. Also interacts with p35 which activates the kinase.,similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.,similarity:Contains 1 protein kinase domain.,subcellular location:In axonal growth cone with extension to the peripheral lamellipodia.,subunit:Heterodimer of a catalytic subunit and a regulatory subunit (p35). Found in a trimolecular complex with CABLES1 and ABL1. Interacts with CABLES1 (By similarity). Interacts with AATK.,
纯化
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
