产品名称
SPHK1 (Phospho-Ser225) Rabbit Polyclonal Antibody
别名
Sphingosine kinase 1 (SK 1) (SPK 1) (EC 2.7.1.91)
蛋白名称
SPHK1 (Phospho-Ser225)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=8877
Human Swissprot No.
Q9NYA1
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q9NYA1/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/20698
Mouse Swissprot No.
Q8CI15
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q8CI15
Rat Gene Link
https://www.uniprot.org/uniprot/170897
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/Q91V26
特异性
This antibody detects endogenous levels of SPHK1 (Phospho-Ser225) at Human, Mouse,Rat
运输及保存条件
-15°C to -25°C/1 year(Do not lower than -25°C)
组织表达
Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle. Expressed in brain cortex (at protein level) (PubMed:29662056).
细胞定位
Cytoplasm . Nucleus . Cell membrane . Endosome membrane ; Peripheral membrane protein . Membrane, clathrin-coated pit . Cell junction, synapse . Translocated from the cytoplasm to the plasma membrane in a CIB1-dependent manner (PubMed:19854831). Binds to membranes containing negatively charged lipids but not neutral lipids (PubMed:24929359). Recruited to endocytic membranes by sphingosine where promotes membrane fusion (By similarity). .
功能
catalytic activity:ATP + sphinganine = ADP + sphinganine 1-phosphate.,catalytic activity:ATP + sphingosine = ADP + sphingosine 1-phosphate.,cofactor:Magnesium.,function:Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra-and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol.,similarity:Contains 1 DAGKc domain.,subunit:Interacts with ACY1 (By similarity). Binds to calmodulin. Interacts with SPHKAP.,tissue specificity:Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle.,
纯化
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
