BD-PP1673

NFAT1 (Phospho-Ser326) Rabbit Polyclonal Antibody

Nuclear factor of activated T-cells, cytoplasmic 2 (NF-ATc2) (NFATc2) (NFAT pre-existing subunit) (NF-ATp) (T-cell transcription factor NFAT1)

常规抗体

NFATC2 NFAT1 NFATP

NFAT1 (Phospho-Ser326)

WB

Human,Mouse,Rat

1 mg/ml

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

4773

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=4773

Q13469

https://www.uniprot.org/uniprotkb/Q13469/entry

18019

https://www.uniprot.org/uniprot/18019

Q60591

https://www.uniprot.org/uniprotkb/Q60591

https://www.uniprot.org/uniprotkb/0

This antibody detects endogenous levels of NFAT1 (Phospho-Ser326) at Human, Mouse,Rat

WB 1:500-2000

100kD

-15°C to -25°C/1 year(Do not lower than -25°C)

Expressed in thymus, spleen, heart, testis, brain, placenta, muscle and pancreas. Isoform 1 is highly expressed in the small intestine, heart, testis, prostate, thymus, placenta and thyroid. Isoform 3 is highly expressed in stomach, uterus, placenta, trachea and thyroid.

Cytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.

alternative products:Additional isoforms seem to exist,domain:Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.,function:Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF.,induction:Inducibly expressed in T-lymphocytes upon activation of the T-cell receptor (TCR) complex. Induced after co-addition of phorbol 12-myristate 13-acetate (PMA) and ionomycin.,PTM:In resting cells, phosphorylated by NFATC-kinase on at least 18 sites in the 99-363 region. Upon cell stimulation, all these sites except Ser-243 are dephosphorylated by calcineurin. Dephosphorylation induces a conformational change that simultaneously exposes an NLS and masks an NES, which results in nuclear localization. Simultaneously, Ser-53 or Ser-56 is phosphorylated; which is required for full transcriptional activity.,similarity:Contains 1 RHD (Rel-like) domain.,subcellular location:Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.,subunit:Member of the multicomponent NFATC transcription complex that consists of at least two components, a pre-existing cytoplasmic component NFATC2 and an inducible nuclear component NFATC1. Other members such as NFATC4, NFATC3 or members of the activating protein-1 family, MAF, GATA4 and Cbp/p300 can also bind the complex. The phosphorylated form specifically interacts with XPO1; which mediates nuclear export. NFATC proteins bind to DNA as monomers. Interacts with NFATC2IP.,tissue specificity:Expressed in thymus, spleen, heart, testis, brain, placenta, muscle and pancreas.,

现货

The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.