产品名称
ASK1 (Phospho-Thr838) Rabbit Polyclonal Antibody
别名
Mitogen-activated protein kinase kinase kinase 5 (EC 2.7.11.25) (Apoptosis signal-regulating kinase 1) (ASK-1) (MAPK/ERK kinase kinase 5) (MEK kinase 5) (MEKK 5)
基因名称
MAP3K5 ASK1 MAPKKK5 MEKK5
蛋白名称
ASK1 (Phospho-Thr838)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=4217
Human Swissprot No.
Q99683
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q99683/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/26408
Mouse Swissprot No.
O35099
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/O35099
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/0
特异性
This antibody detects endogenous levels of ASK1 (Phospho-Thr838) at Human, Mouse,Rat
运输及保存条件
-15°C to -25°C/1 year(Do not lower than -25°C)
组织表达
Abundantly expressed in heart and pancreas.
细胞定位
Cytoplasm . Endoplasmic reticulum. Interaction with 14-3-3 proteins alters the distribution of MAP3K5/ASK1 and restricts it to the perinuclear endoplasmic reticulum region.
功能
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Contains an N-terminal autoinhibitory domain. Activated by phosphorylation at Thr-838, inhibited by phosphorylation at Ser-966 and Ser-1033. Binds to, and stabilizes MAP3K6 and is activated by MAP3K6 by phosphorylation on Thr-838.,function:Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death.,induction:By TNF-alpha. Inhibited by HIV-1 Nef.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Homodimer when inactive. Binds both upstream activators and downstream substrates in multimolecular complexes. Associates with and inhibited by HIV-1 Nef. Interacts with DAB2IP and PPM1L.,tissue specificity:Abundantly expressed in heart and pancreas.,
纯化
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
