产品名称
IRE1α Rabbit Polyclonal Antibody
别名
ERN1; IRE1; Serine/threonine-protein kinase/endoribonuclease IRE1; Endoplasmic reticulum-to-nucleus signaling 1; Inositol-requiring protein 1; hIRE1p; Ire1-alpha; IRE1a
蛋白名称
Serine/threonine-protein kinase/endoribonuclease IRE1
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=2081
Human Swissprot No.
O75460
Human Swissprot Link
http://www.uniprot.org/uniprotkb/O75460/entry
Mouse Swissprot No.
Q9EQY0
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9EQY0
特异性
IRE1α Antibody detects endogenous levels of IRE1α protein.
稀释度
WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.
运输及保存条件
-15°C to -25°C/1 year(Do not lower than -25°C)
组织表达
Ubiquitously expressed. High levels observed in pancreatic tissue.
细胞定位
Endoplasmic reticulum membrane ; Single-pass type I membrane protein .
信号通路
Alzheimer's disease;
功能
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:The kinase domain is activated by trans-autophosphorylation. Kinase activity is required for activation of the endoribonuclease domain.,function:Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.,PTM:Autophosphorylated.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.,similarity:Contains 1 KEN domain.,similarity:Contains 1 protein kinase domain.,subunit:Homodimer; disulfide-linked. Dimer formation is driven by hydrophobic interactions within the N-terminal luminal domains and stabilized by disulfide bridges. Also binds HSPA5, a negative regulator of the unfolded protein response. This interaction may disrupt homodimerization and prevent activation of ERN1.,tissue specificity:Ubiquitously expressed. High levels observed in pancreatic tissue.,
