产品名称
PDE4D Rabbit Polyclonal Antibody
别名
cAMP-specific 3',5'-cyclic phosphodiesterase 4D (EC 3.1.4.17) (DPDE3) (PDE43)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=5144
Human Swissprot No.
Q08499
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q08499/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/238871
Mouse Swissprot No.
Q01063
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q01063
Rat Gene Link
https://www.uniprot.org/uniprot/24627
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/P14270
特异性
This antibody detects endogenous levels of PDE4D at Human, Mouse,Rat
参考分子量
isform D3:76kD, isform D4 91kD, isform D1 D2 66kD
运输及保存条件
-15°C to -25°C/1 year(Do not lower than -25°C)
组织表达
Expressed in colonic epithelial cells (at protein level). Widespread; most abundant in skeletal muscle. ; [Isoform 6]: Detected in brain. ; [Isoform 8]: Detected in brain, placenta, lung and kidney. ; [Isoform 7]: Detected in heart and skeletal muscle.
细胞定位
Apical cell membrane . Cytoplasm . Membrane . Cytoplasm, cytoskeleton . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Found in the soluble fraction, associated with membranes, and associated with the cytoskeleton and the centrosome (By similarity). Colocalized with SHANK2 to the apical membrane of colonic crypt cells. .
功能
catalytic activity:Adenosine 3',5'-cyclic phosphate + H(2)O = adenosine 5'-phosphate.,cofactor:Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.,disease:Genetic variations in PDE4D might be associated with susceptibility to stroke type 1 (STRK1) [MIM:606799]. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. PubMed:17006457 states that association with stroke has to be considered with caution.,enzyme regulation:Inhibited by rolipram. Activated by phosphatidic acid.,function:Regulates the levels of cAMP in the cell.,pathway:Purine metabolism; cAMP degradation; AMP from cAMP: step 1/1.,PTM:Isoform 2 and isoform 11 are activated by phosphorylation (in vitro), but not isoform 8. Isoform 7 and isoform 12 are phosphorylated on Ser-49, Ser-51, Ser-55 and Ser-59.,similarity:Belongs to the cyclic nucleotide phosphodiesterase family.,subcellular location:Found in the soluble fraction, associated with membranes, and associated with the cytoskeleton and the centrosome.,subunit:Homodimer for the long isoforms. Isoforms with truncated N-termini are monomeric. Isoform 2 is part of a ternary complex containing PRKAR2A, PRKAR2B and AKAP9. Interacts with PDE4DIP (By similarity). Isoform 5 binds GNB2L1 via its unique N-terminus. Binds ARRB2.,tissue specificity:Widespread; most abundant in skeletal muscle. Isoform 8 is detected in brain. Isoform 9 is detected in brain, placenta, lung and kidney. Isoform 11 is detected in heart and skeletal muscle.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
