产品名称
LC3 A/B Rabbit Polyclonal Antibody
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Swissprot No.
Q9H492/Q9GZQ8
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q9H492/entry
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/
免疫原
Synthesized peptide derived from human LC3 A/B
特异性
This antibody detects endogenous levels of Human,Mouse,Rat LC3 A/B
稀释度
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
function:Probably involved in formation of autophagosomal vacuoles (autophagosomes).,PTM:The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.,similarity:Belongs to the MAP1 LC3 family.,subcellular location:LC3-II binds to the autophagic membranes.,subunit:3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins.,tissue specificity:Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.,
组织表达
Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.
细胞定位
Cytoplasmic vesicle, autophagosome membrane ; Lipid-anchor . Endomembrane system ; Lipid-anchor . Cytoplasm, cytoskeleton . LC3-II binds to the autophagic membranes. .
功能
autophagic vacuole formation,?proteolysis,?autophagy,?vacuole organization,?macromolecule catabolic process,?cellular response to starvation,?response to extracellular stimulus,?macroautophagy,?modification-dependent protein catabolic process,?protein catabolic process,?response to nutrient levels,?cellular response to extracellular stimulus,?cellular response to nutrient levels,?cellular response to stress,?response to starvation,?modification-dependent macromolecule catabolic process,?cellular protein catabolic process,?cellular macromolecule catabolic process,proteolysis involved in cellular protein catabolic process,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
