产品名称
RIP140 Rabbit Polyclonal Antibody
别名
Nuclear receptor-interacting protein 1 (Nuclear factor RIP140;Receptor-interacting protein 140)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/8204
Human Swissprot No.
P48552
Human Swissprot Link
https://www.uniprot.org/uniprotkb/P48552/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/268903
Mouse Swissprot No.
Q8CBD1
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q8CBD1
免疫原
Synthesized peptide derived from human RIP140
特异性
This antibody detects endogenous levels of Human,Mouse,Rat RIP140
稀释度
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
Nuclear receptor interacting protein 1 (NRIP1) is a nuclear protein that specifically interacts with the hormone-dependent activation domain AF2 of nuclear receptors. Also known as RIP140, this protein modulates transcriptional activity of the estrogen receptor. [provided by RefSeq, Jul 2008],
细胞定位
Nucleus . Localized to discrete foci and redistributes to larger nuclear domains upon binding to ligand-bound NR3C1.
功能
disease:Genetic variation in NRIP1 may act as predisposing factor for endometriosis.,domain:Contains 9 Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs, which have different affinities for nuclear receptors. The C-terminal LTKTNPILYYMLQK motif is required for ligand-dependent interaction with RAAR and RXRB homo- and heterodimers, for the corepressor activity, and for the formation of an HDAC3 complex with RARA/RXRB (By similarity). Contains at least four autonomous repression domains (RD1-4). RD1 functions via a histone deacetylase (HDAC)-independent mechanism, whereas RD2, RD3 and RD4 can function by HDAC-dependent or independent mechanisms, depending on cell type. RD2 is dependent on CTBP binding.,function:Modulates transcriptional activation by steroid receptors such as NR3C1, NR3C2 and ESR1. Also modulates transcriptional repression by nuclear hormone receptors.,PTM:Acetylation regulates its nuclear translocation and corepressive activity (By similarity). Acetylation abolishes interaction with CTBP1. Phosphorylation enhances interaction with YWHAH.,subcellular location:Localized to discrete foci and redistributes to larger nuclear domains upon binding to ligand-bound NR3C1.,subunit:Interacts with the ligand binding domain (LBD) of NR2C1 in the absence of ligand. Interacts with RARA and RXRB homodimers and RARA/RXRB heterodimers in the presence of ligand. Interacts with HDAC1 and HDAC3 via its N-terminal domain (By similarity). Interacts with CTBP1, CTBP2, ESR1, HDAC1, HDAC2, HDAC5, HDAC6, NR3C1, NR3C2, YWHAH, JUN and FOS. Found in a complex with both NR3C1 and YWHAH.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
