产品名称
HMGN1/2/3/4 Rabbit Polyclonal Antibody
别名
Non-histone chromosomal protein HMG-14 (High mobility group nucleosome-binding domain-containing protein 1)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/3150
Human Swissprot No.
P05114/P05204/Q15651/O00479
Human Swissprot Link
https://www.uniprot.org/uniprotkb/P05114/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/100044391
Mouse Swissprot No.
P18608
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/P18608
免疫原
Synthesized peptide derived from human HMGN1/2/3/4 AA range: 1-50
特异性
This antibody detects endogenous levels of Human,Mouse,Rat HMGN1/2/3/4
稀释度
WB 1:1000-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene binds nucleosomal DNA and is associated with transcriptionally active chromatin. Along with a similar protein, HMG17, the encoded protein may help maintain an open chromatin configuration around transcribable genes. [provided by RefSeq, Aug 2011],
细胞定位
Nucleus. Cytoplasm. Cytoplasmic enrichment upon phosphorylation. The RNA edited version localizes to the nucleus.
功能
function:Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in an unique chromatin conformation. Inhibits the phosphorylation of nucleosomal histones H3 and H2A by RPS6KA5/MSK1 and RPS6KA3/RSK2.,mass spectrometry: PubMed:10739259,PTM:Phosphorylation on Ser-21 and Ser-25 weakens binding to nucleosomes and increases the rate of H3 phosphorylation (By similarity). Phosphorylation favors cytoplasmic localization.,RNA editing:Partially edited. A new initiator methionine may be created by a single uridine insertion in the 5'-UTR, causing an N-terminal extension of 45 amino acids. The existence of the RNA edited version is supported by direct protein sequencing by MS/MS of the following peptides specific to that version: 23-31 and 40-48. The RNA edited version is called ET-HMGN1.,similarity:Belongs to the HMGN family.,subcellular location:Cytoplasmic enrichment upon phosphorylation. The RNA edited version localizes to the nucleus.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
