产品名称
ATG5 Rabbit Polyclonal Antibody
别名
Autophagy protein 5 (APG5-like;Apoptosis-specific protein)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/9474
Human Swissprot No.
Q9H1Y0
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q9H1Y0/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/11793
Mouse Swissprot No.
Q99J83
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q99J83
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/Q3MQ06
免疫原
Synthesized peptide derived from human ATG5 AA range: 180-260
特异性
This antibody detects endogenous levels of Human,Mouse,Rat ATG5
稀释度
WB 1:1000-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Sep 2015],
组织表达
Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells.
细胞定位
Cytoplasm . Preautophagosomal structure membrane; Peripheral membrane protein. Colocalizes with nonmuscle actin. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (By similarity). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme. .
功能
function:May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.,function:Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed.,induction:By apoptotic stimuli.,PTM:Conjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane.,similarity:Belongs to the ATG5 family.,subcellular location:Colocalizes with nonmuscle actin.,tissue specificity:Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
