产品名称
Proteasome α3 Rabbit Polyclonal Antibody
别名
Proteasome subunit alpha type-3 (EC 3.4.25.1;Macropain subunit C8;Multicatalytic endopeptidase complex subunit C8;Proteasome component C8)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/5684
Human Swissprot No.
P25788
Human Swissprot Link
https://www.uniprot.org/uniprotkb/P25788/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/19167
Mouse Swissprot No.
O70435
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/O70435
Rat Gene Link
https://www.uniprot.org/uniprot/29670
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/P18422
免疫原
Synthesized peptide derived from human Proteasome α3 AA range: 190-270
特异性
This antibody detects endogenous levels of Human,Mouse,Rat Proteasome α3
稀释度
WB 1:500-2000;IHC-p 1:50-300
宿主
Polyclonal, Rabbit,IgG
背景介绍
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq, Jul 2008],
细胞定位
Cytoplasm . Nucleus . Translocated from the cytoplasm into the nucleus following interaction with AKIRIN2, which bridges the proteasome with the nuclear import receptor IPO9. .
功能
catalytic activity:Cleavage of peptide bonds with very broad specificity.,function:The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.,similarity:Belongs to the peptidase T1A family.,subunit:The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits.,subunit:The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
