产品名称
PDHK1 (Phospho Tyr243) Rabbit Polyclonal Antibody
别名
[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial (EC 2.7.11.2;Pyruvate dehydrogenase kinase isoform 1)
蛋白名称
PDHK1 (Phospho Tyr243)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/5163
Human Swissprot No.
Q15118
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q15118/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/228026
Mouse Swissprot No.
Q8BFP9
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q8BFP9
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/Q63065
免疫原
Synthesized peptide derived from human PDHK1 (Phospho Tyr243)
特异性
This antibody detects endogenous levels of Human,Mouse,Rat PDHK1 (Phospho Tyr243)
稀释度
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2013],
组织表达
Expressed predominantly in the heart. Detected at lower levels in liver, skeletal muscle and pancreas.
细胞定位
Mitochondrion matrix .
功能
catalytic activity:ATP + [pyruvate dehydrogenase (acetyl-transferring)] = ADP + [pyruvate dehydrogenase (acetyl-transferring)] phosphate.,function:Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.,similarity:Belongs to the PDK/BCKDK protein kinase family.,similarity:Contains 1 histidine kinase domain.,tissue specificity:Expressed predominantly in the heart.,
纯化
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
