产品名称
TOP1M Rabbit Polyclonal Antibody
蛋白名称
DNA topoisomerase I, mitochondrial (TOP1mt) (EC 5.99.1.2)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
Q969P6
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q969P6/entry
Mouse Swissprot No.
Q8R4U6
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q8R4U6
Rat Swissprot Link
http://www.uniprot.org/uniprot/O54941Q6IM78
免疫原
Synthesized peptide derived from part region of human protein
特异性
TOP1M Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a mitochondrial DNA topoisomerase that plays a role in the modification of DNA topology. The encoded protein is a type IB topoisomerase and catalyzes the transient breaking and rejoining of DNA to relieve tension and DNA supercoiling generated in the mitochondrial genome during replication and transcription. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012],
组织表达
Ubiquitous; highest in skeletal muscle, heart, brain and fetal liver.
功能
catalytic activity:ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.,cofactor:Divalent metal ions (calcium or magnesium).,function:Relieves DNA strain that arise during duplication of mitochondrial DNA.,miscellaneous:When a topoisomerase transiently breaks a DNA backbone bond, it simultaneously forms a protein-DNA link, in which a tyrosyl oxygen in the enzyme is joined to a DNA phosphorus at one end of the enzyme-severed DNA strand.,similarity:Belongs to the eukaryotic type I topoisomerase family.,tissue specificity:Ubiquitous; highest in skeletal muscle, heart, brain and fetal liver.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
