产品名称
Calpain 2 (Phospho Ser50) Rabbit Polyclonal Antibody
别名
Calpain-2 catalytic subunit (EC 3.4.22.53;Calcium-activated neutral proteinase 2;CANP 2;Calpain M-type;Calpain large polypeptide L2;Calpain-2 large subunit;Millimolar-calpain;M-calpain)
蛋白名称
Calpain 2 (Phospho Ser50)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/824
Human Swissprot No.
P17655
Human Swissprot Link
https://www.uniprot.org/uniprotkb/P17655/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/12334
Mouse Swissprot No.
O08529
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/O08529
Rat Gene Link
https://www.uniprot.org/uniprot/29154
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/Q07009
免疫原
Synthesized peptide derived from human Calpain 2 (Phospho Ser50)
特异性
This antibody detects endogenous levels of Human,Mouse,Rat Calpain 2 (Phospho Ser50)
稀释度
WB 1:1000-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009],
细胞定位
Cytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding.
功能
catalytic activity:Broad endopeptidase specificity.,cofactor:Binds 3 calcium ions.,enzyme regulation:Activated by 200-1000 micromolar concentrations of calcium and inhibited by calpastatin.,function:Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.,sequence caution:Contaminating sequence. Potential poly-A sequence.,similarity:Belongs to the peptidase C2 family.,similarity:Contains 1 calpain catalytic domain.,similarity:Contains 3 EF-hand domains.,subcellular location:Translocates to the plasma membrane upon Ca(2+) binding.,subunit:Forms a heterodimer with a small (regulatory) subunit (CAPNS1).,tissue specificity:Ubiquitous.,
纯化
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
