产品名称
TOP2A (Phospho Ser1213) Rabbit Polyclonal Antibody
别名
DNA topoisomerase 2-alpha (EC 5.99.1.3;DNA topoisomerase II, alpha isozyme)
蛋白名称
TOP2A (Phospho Ser1213)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/7153
Human Swissprot No.
P11388
Human Swissprot Link
https://www.uniprot.org/uniprotkb/P11388/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/21973
Mouse Swissprot No.
Q01320
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q01320
Rat Gene Link
https://www.uniprot.org/uniprot/360243
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/P41516
免疫原
Synthesized peptide derived from human TOP2A (Phospho Ser1213)
特异性
This antibody detects endogenous levels of Human TOP2A (Phospho Ser1213)
稀释度
WB 1:1000-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
catalytic activity:ATP-dependent breakage, passage and rejoining of double-stranded DNA.,enzyme regulation:Specifically inhibited by the intercalating agent amsacrine.,function:Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.,miscellaneous:Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils.,PTM:Phosphorylation has no effect on catalytic activity.,similarity:Belongs to the type II topoisomerase family.,subcellular location:Generally located in the nucleoplasm.,subunit:Homodimer. Interacts with COPS5.,
组织表达
Expressed in the tonsil, spleen, lymph node, thymus, skin, pancreas, testis, colon, kidney, liver, brain and lung (PubMed:9155056). Also found in high-grade lymphomas, squamous cell lung tumors and seminomas (PubMed:9155056).
细胞定位
Cytoplasm . Nucleus, nucleoplasm . Nucleus . Nucleus, nucleolus .
功能
DNA metabolic process,?DNA replication,?DNA topological change,?DNA ligation,?DNA repair,?DNA packaging,?apoptosis,response to DNA damage stimulus,?nucleus organization,?chromosome segregation,?intracellular signaling cascade,?cell death,?regulation of cell death,?positive regulation of cell death,?programmed cell death,?death,?second-messenger-mediated signaling,?chromosome condensation,?apoptotic nuclear changes,?apoptotic chromosome condensation,cellular response to stress,?regulation of apoptosis,?positive regulation of apoptosis,?regulation of programmed cell death,?positive regulation of programmed cell death,?regulation of viral genome replication,?positive regulation of viral genome replication,?regulation of retroviral genome replication,?positive regulation of retroviral genome replication,phosphoinositide-mediated signaling,?positive regulation of viral reproduction,?regulation of viral reproduction,chromosome organization,
纯化
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
