产品名称
MLK3 (Phospho Thr277+Ser281) Rabbit Polyclonal Antibody
别名
Mitogen-activated protein kinase kinase kinase 11 (EC 2.7.11.25;Mixed lineage kinase 3;Src-homology 3 domain-containing proline-rich kinase)
基因名称
MAP3K11 MLK3 PTK1 SPRK
蛋白名称
MLK3 (Phospho Thr277+Ser281)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/4296
Human Swissprot No.
Q16584
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q16584/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/26403
Mouse Swissprot No.
Q80XI6
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q80XI6
Rat Gene Link
https://www.uniprot.org/uniprot/309168
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/Q66HA1
免疫原
Synthesized peptide derived from human MLK3 (Phospho Thr277+Ser281)
特异性
This antibody detects endogenous levels of Human,Mouse,Rat MLK3 (Phospho Thr277+Ser281)
稀释度
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene is a member of the serine/threonine kinase family. This kinase contains a SH3 domain and a leucine zipper-basic motif. This kinase preferentially activates MAPK8/JNK kinase, and functions as a positive regulator of JNK signaling pathway. This kinase can directly phosphorylate, and activates IkappaB kinase alpha and beta, and is found to be involved in the transcription activity of NF-kappaB mediated by Rho family GTPases and CDC42. [provided by RefSeq, Jul 2008],
组织表达
Expressed in a wide variety of normal and neoplastic tissues including fetal lung, liver, heart and kidney, and adult lung, liver, heart, kidney, placenta, skeletal muscle, pancreas and brain.
细胞定位
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Location is cell cycle dependent.
功能
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Homodimerization via the leucine zipper domains is required for autophosphorylation and subsequent activation.,function:Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1). Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle.,PTM:Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-277 is likely to be the main autophosphorylation site. Phosphorylation of Ser-555 and Ser-556 is induced by CDC42.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SH3 domain.,subcellular location:Location is cell cycle dependent.,subunit:Homodimer; undergoes dimerization during activation.,tissue specificity:Expressed in a wide variety of normal and neoplastic tissues including fetal lung, liver, heart and kidney, and adult lung, liver, heart, kidney, placenta, skeletal muscle, pancreas and brain.,
纯化
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
