产品名称
BACE (Phospho Ser498) Rabbit Polyclonal Antibody
别名
Beta-secretase 1 (EC 3.4.23.46;Aspartyl protease 2;ASP2;Asp 2;Beta-site amyloid precursor protein cleaving enzyme 1;Beta-site APP cleaving enzyme 1;Memapsin-2;Membrane-associated aspartic protease 2)
蛋白名称
BACE (Phospho Ser498)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/23621
Human Swissprot No.
P56817
Human Swissprot Link
https://www.uniprot.org/uniprotkb/P56817/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/23821
Mouse Swissprot No.
P56818
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/P56818
Rat Gene Link
https://www.uniprot.org/uniprot/29392
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/P56819
免疫原
Synthesized peptide derived from human BACE (Phospho Ser498)
特异性
This antibody detects endogenous levels of Human,Mouse,Rat BACE (Phospho Ser498)
稀释度
WB 1:1000-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
beta-secretase 1(BACE1) Homo sapiens This gene encodes a member of the peptidase A1 family of aspartic proteases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protease. This transmembrane protease catalyzes the first step in the formation of amyloid beta peptide from amyloid precursor protein. Amyloid beta peptides are the main constituent of amyloid beta plaques, which accumulate in the brains of human Alzheimer's disease patients. [provided by RefSeq, Nov 2015],
组织表达
Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata.
细胞定位
Cell membrane ; Single-pass type I membrane protein . Golgi apparatus, trans-Golgi network . Endoplasmic reticulum . Endosome . Cell surface . Cytoplasmic vesicle membrane ; Single-pass type I membrane protein . Membrane raft . Lysosome . Late endosome . Early endosome . Recycling endosome . Cell projection, axon . Cell projection, dendrite . Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface (PubMed:17425515, PubMed:11466313). Colocalization with APP in early endosomes is due to addition of bisecting N-acetylglucosamine wich blocks targeting to late endosomes and lysosomes (By similarity). Retrogradly transported from endosomal compartments to the trans-Golgi network in a phosphorylation- and GGA1- dependent manner (PubMed:15886016). .
功能
catalytic activity:Broad endopeptidase specificity. Cleaves Glu-Val-Asn-Leu-|-Asp-Ala-Glu-Phe in the Swedish variant of Alzheimer's amyloid precursor protein.,enzyme regulation:Inhibited by RTN3 and RTN4.,function:Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase.,similarity:Belongs to the peptidase A1 family.,subunit:Monomer. Interacts with GGA1, GGA2 and GGA3. Interacts with RTN3 and RTN4.,tissue specificity:Brain.,
纯化
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
