产品名称
RPA1 Rabbit Polyclonal Antibody
蛋白名称
DNA-directed RNA polymerase I subunit RPA1 (RNA polymerase I subunit A1) (EC 2.7.7.6) (A190) (DNA-directed RNA polymerase I largest subunit) (DNA-directed RNA polymerase I subunit A) (RNA polymerase I 194 kDa subunit) (RPA194)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
O95602
Human Swissprot Link
https://www.uniprot.org/uniprotkb/O95602/entry
Mouse Swissprot No.
O35134
Mouse Swissprot Link
http://www.uniprot.org/uniprot/O35134
Rat Swissprot Link
http://www.uniprot.org/uniprot/O54941O54889
免疫原
Synthesized peptide derived from part region of human protein
特异性
RPA1 Polyclonal Antibody detects endogenous levels of protein.
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene is the largest subunit of the RNA polymerase I complex. The encoded protein represents the catalytic subunit of the complex, which transcribes DNA into ribosomal RNA precursors. Defects in this gene are a cause of the Cincinnati type of acrofacial dysostosis. [provided by RefSeq, May 2016],
细胞定位
Nucleus, nucleolus . Chromosome .
信号通路
Purine metabolism;Pyrimidine metabolism;RNA polymerase;
功能
catalytic activity:Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).,function:DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol I. A bridging helix emanates from RPA1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol I by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition.,PTM:Phosphorylated.,similarity:Belongs to the RNA polymerase beta' chain family.,subunit:Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits. Interacts with MYO1C.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
