产品名称
PA24B Rabbit Polyclonal Antibody
蛋白名称
Cytosolic phospholipase A2 beta (cPLA2-beta) (EC 3.1.1.4) (Phospholipase A2 group IVB)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
P0C869
Human Swissprot Link
https://www.uniprot.org/uniprotkb/P0C869/entry
Mouse Swissprot No.
P0C871
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P0C871
免疫原
Synthesized peptide derived from part region of human protein
特异性
PA24B Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
This locus represents naturally-occurring readthrough transcription between the neighboring jumonji domain containing 7 (JMJD7) and phospholipase A2, group IVB (cytosolic) (PLA2G4B) genes. Readthrough transcripts encode fusion proteins that share amino acid sequence with each individual gene product, including a partial JmjC domain and downstream C2 and phospholipase A2 domains. Alternatively spliced transcript variants have been observed. [provided by RefSeq, Oct 2013],
组织表达
Widely expressed. Expressed at higher level in brain, heart, liver, cerebellum and pancreas.
细胞定位
[Isoform 3]: Cytoplasm, cytosol . Mitochondrion membrane ; Peripheral membrane protein. Early endosome membrane ; Peripheral membrane protein. Translocates to membrane vesicles in a calcium-dependent fashion. .; [Isoform 5]: Cytoplasm, cytosol .
信号通路
Glycerophospholipid metabolism;Ether lipid metabolism;Arachidonic acid metabolism;Linoleic acid metabolism;alpha-Linolenic acid metabolism;MAPK_ERK_Growth;MAPK_G_Protein;Vascular smooth muscle contraction;VEGF;Fc epsilon RI;Fc gamma R-mediated phagocytosi
功能
catalytic activity:Phosphatidylcholine + H(2)O = 1-acylglycerophosphocholine + a carboxylate.,caution:Most tissues also express read-through transcripts from this gene into the upstream gene (Jmjd7), some of which may encode fusion proteins.,caution:This sequence was first thought to be an alternatively spliced isoform of Pla2g4b. It is derived from Jmjd7 which is located upstream of Pla2g4b. Most tissues also express read-through transcripts from Jmjd7 into the downstream Pla2g4b gene, some of which may encode fusion proteins combining the N-terminus of this protein with PLA2G4B protein.,domain:The N-terminal C2 domain associates with lipid membranes and mediates its regulation by presenting the active site to its substrate in response to elevations of cytosolic Ca(2+).,enzyme regulation:Stimulated by cytosolic Ca(2+).,function:Calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position with a preference for arachidonoyl phospholipids. Has a much weaker activity than PLA2G4A. Isoform 3 has calcium-dependent activity against palmitoyl-arachidonyl-phosphatidylethanolamine and low level lysophospholipase activity but no activity against phosphatidylcholine.,similarity:Contains 1 C2 domain.,similarity:Contains 1 JmjC domain.,similarity:Contains 1 PLA2c domain.,subcellular location:Translocates to membrane vesicles in a calcium-dependent fashion.,tissue specificity:Widely expressed. Expressed at higher level in brain, heart, liver, cerebellum and pancreas. Isoform 3 is widely expressed.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
