产品名称
NCK1 Rabbit Polyclonal Antibody
蛋白名称
Cytoplasmic protein NCK1 (NCK adaptor protein 1) (Nck-1) (SH2/SH3 adaptor protein NCK-alpha)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
P16333
Human Swissprot Link
https://www.uniprot.org/uniprotkb/P16333/entry
Mouse Swissprot No.
Q99M51
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q99M51
免疫原
Synthesized peptide derived from part region of human protein
特异性
NCK1 Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Jun 2010],
组织表达
Epithelium,Platelet,Uterus,
细胞定位
Cytoplasm. Endoplasmic reticulum. Nucleus. Mostly cytoplasmic, but shuttles between the cytoplasm and the nucleus. Import into the nucleus requires the interaction with SOCS7. Predominantly nuclear following genotoxic stresses, such as UV irradiation, hydroxyurea or mitomycin C treatments.
信号通路
ErbB_HER;Axon guidance;T_Cell_Receptor;Pathogenic Escherichia coli infection;
功能
function:Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1.,PTM:Phosphorylated on Ser and Tyr residues.,similarity:Contains 1 SH2 domain.,similarity:Contains 3 SH3 domains.,subunit:Associates with BLNK, PLCG1, VAV1 and NCK1 in a B-cell antigen receptor-dependent fashion. Interacts with SOCS7. Part of a complex containing PPP1R15B, PP1 and NCK1. Interacts with RALGPS1.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
