产品名称
DHI2 Rabbit Polyclonal Antibody
蛋白名称
Corticosteroid 11-beta-dehydrogenase isozyme 2 (EC 1.1.1.-) (11-beta-hydroxysteroid dehydrogenase type 2) (11-DH2) (11-beta-HSD2) (11-beta-hydroxysteroid dehydrogenase type II) (-HSD11 type II) (NAD-dependent 11-beta-hydroxysteroid dehydrogenase) (11-beta-HSD)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
P80365
Human Swissprot Link
https://www.uniprot.org/uniprotkb/P80365/entry
Mouse Swissprot No.
P51661
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P51661
Rat Swissprot Link
http://www.uniprot.org/uniprot/O54941P50233
免疫原
Synthesized peptide derived from part region of human protein
特异性
DHI2 Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
hydroxysteroid 11-beta dehydrogenase 2(HSD11B2) Homo sapiens There are at least two isozymes of the corticosteroid 11-beta-dehydrogenase, a microsomal enzyme complex responsible for the interconversion of cortisol and cortisone. The type I isozyme has both 11-beta-dehydrogenase (cortisol to cortisone) and 11-oxoreductase (cortisone to cortisol) activities. The type II isozyme, encoded by this gene, has only 11-beta-dehydrogenase activity. In aldosterone-selective epithelial tissues such as the kidney, the type II isozyme catalyzes the glucocorticoid cortisol to the inactive metabolite cortisone, thus preventing illicit activation of the mineralocorticoid receptor. In tissues that do not express the mineralocorticoid receptor, such as the placenta and testis, it protects cells from the growth-inhibiting and/or pro-apoptotic effects of cortisol, particularly during embryonic development. Mutations in this gene cause the syndrome of apparent mine
组织表达
Expressed in kidney, placenta, pancreas, prostate, ovary, small intestine and colon, and in lower levels in the spleen and testis (PubMed:7859916). At midgestation, expressed at high levels in placenta and in fetal kidney and, at much lower levels, in fetal lung and testis (PubMed:8530071).
细胞定位
Microsome . Endoplasmic reticulum .
信号通路
Steroid hormone biosynthesis;Androgen and estrogen metabolism;Aldosterone-regulated sodium reabsorption;
功能
catalytic activity:An 11-beta-hydroxysteroid + NAD(+) = an 11-oxosteroid + NADH.,disease:Defects in HSD11B2 are the cause of apparent mineralocorticoid excess (AME) [MIM:218030]. AME is a potentially fatal disease characterized by severe juvenile low-renin hypertension, sodium retention, hypokalemia and low levels of aldosterone. It often leads to nephrocalcinosis.,enzyme regulation:Inhibited by glycyrrhetinic acid (derived from liquorice), carbenoloxone and 11-alpha-OH-progesterone.,function:Catalyzes the conversion of cortisol to the inactive metabolite cortisone. Modulates intracellular glucocorticoid levels, thus protecting the nonselective mineralocorticoid receptor from occupation by glucocorticoids.,miscellaneous:Consumption of large amounts of liquorice can lead to apparent mineralocorticoid excess and hypertension.,similarity:Belongs to the short-chain dehydrogenases/reductases (SDR) family.,subunit:Interacts with ligand-free cytoplasmic NR3C2.,tissue specificity:Found in placenta, kidney, pancreas, prostate, ovary, small intestine and colon.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
