产品名称
EKLF (Acetyl Lys274) Rabbit Polyclonal Antibody
别名
Krueppel-like factor 1 (Erythroid krueppel-like transcription factor;EKLF)
蛋白名称
EKLF (Acetyl Lys274)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/10661
Human Swissprot No.
Q13351
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q13351/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/0
Mouse Swissprot No.
P46099
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/P46099
免疫原
Synthesized peptide derived from human EKLF (Acetyl Lys274)
特异性
This antibody detects endogenous levels of Human EKLF (Acetyl Lys274)
稀释度
WB 1:1000-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a hematopoietic-specific transcription factor that induces high-level expression of adult beta-globin and other erythroid genes. The zinc-finger protein binds to the DNA sequence CCACACCCT found in the beta hemoglobin promoter. Heterozygous loss-of-function mutations in this gene result in the dominant In(Lu) blood phenotype. [provided by RefSeq, Oct 2009],
组织表达
Expression restricted to adult bone marrow and fetal liver. Not expressed in myeloid nor lymphoid cell lines.
细胞定位
Nucleus . Colocalizes with SUMO1 in nuclear speckles. .
功能
function:Transcription regulator of erythrocyte development. Binds to the CACCC box in the beta-globin gene promoter and activates transcription. When sumoylation, acts as a Probably serves as a general switch factor for erythroid development. When sumoylated, acts as a transcriptional repressor, by promoting interaction with CDH2/MI2beta and also represses megakaryocytic differentiation.,PTM:Acetylated; can be acetylated on both Lys-274 and Lys-288. Acetylation on Lys-274 (by CBP) appears to be the major site affecting EKLF transactivation activity.,PTM:Phosphorylated primarily on serine residues in the transactivation domain. Phosphorylation on Thr-23 is critical for the transactivation activity.,PTM:Sumoylated; sumoylation, promoted by PIAS1, leads to repression of megakaryocyte differentiation. Also promotes the interaction with the CDH4 subunit of the NuRD repression complex.,similarity:Belongs to the krueppel C2H2-type zinc-finger protein family.,similarity:Contains 3 C2H2-type zinc fingers.,subcellular location:Colocalizes with SUMO1 in nuclear speckles.,subunit:Interacts with CBP and EP300; the interactions enhance the transactivation activity. Interacts with PCAF; the interaction does not acetylate EKLF and inhibits its transactivation activity.,tissue specificity:Expression restricted to adult bone marrow and fetal liver. Not expressed in myeloid nor lymphoid cell lines.,
纯化
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
