产品名称
Histone H4 (Tri Methyl Lys21) Rabbit Polyclonal Antibody
基因名称
HIST1H4A H4/A H4FA; HIST1H4B H4/I H4FI; HIST1H4C H4/G H4FG; HIST1H4D H4/B H4FB; HIST1H4E H4/J H4FJ; HIST1H4F H4/C H4FC; HIST1H4H H4/H H4FH; HIST1H4I H4/M H4FM; HIST1H4J H4/E H4FE; HIST1H4K H4/D H4FD; HIST1H4L H4/K H4FK; HIST2H4A H4/N H4F2 H4FN HIST2H4; HI
蛋白名称
Histone H4 (Tri Methyl Lys21)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/121504
Human Swissprot No.
P62805
Human Swissprot Link
https://www.uniprot.org/uniprotkb/P62805/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/100041230
Mouse Swissprot No.
P62806
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/P62806
Rat Gene Link
https://www.uniprot.org/uniprot/100360950
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/P62804
免疫原
Synthesized peptide derived from human Histone H4 (Tri Methyl Lys21)
特异性
This antibody detects endogenous levels of Human,Mouse,Rat Histone H4 (Tri Methyl Lys21)
稀释度
WB 1:1000-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
function:Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.,PTM:Acetylation at Lys-6, Lys-9, Lys-13 and Lys-17 occurs in coding regions of the genome but not in heterochromatin.,PTM:Citrullination at Arg-4 by PADI4 impairs methylation.,PTM:Monomethylated, dimethylated or trimethylated at Lys-21. Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.,PTM:Monomethylation at Arg-4 by PRMT1 favors acetylation at Lys-9 and Lys-13. Demethylation is performed by JMJD6.,PTM:Sumoylated, which is associated with transcriptional repression.,PTM:Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.,similarity:Belongs to the histone H4 family.,subunit:The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.,
细胞定位
Nucleus. Chromosome.
功能
DNA packaging,?chromatin organization,?chromatin assembly or disassembly,?nucleosome assembly,?intracellular signaling cascade,?second-messenger-mediated signaling,?chromatin assembly,?cellular macromolecular complex subunit organization,?cellular macromolecular complex assembly,?nucleosome organization,?macromolecular complex subunit organization,?negative regulation of cell differentiation,?regulation of myeloid cell differentiation,?negative regulation of myeloid cell differentiation,?regulation of megakaryocyte differentiation,?negative regulation of megakaryocyte differentiation,?phosphoinositide-mediated signaling,?chromosome organization,?macromolecular complex assembly,?protein-DNA complex assembly,
纯化
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
