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PRG4 Rabbit Polyclonal Antibody (N-Term)

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产品基本信息

产品货号
BD-PB3748
产品名称
PRG4 Rabbit Polyclonal Antibody (N-Term)
别名
Proteoglycan 4, Lubricin, Megakaryocyte-stimulating factor, Superficial zone proteoglycan, Proteoglycan 4 C-terminal part, PRG4, MSF, SZP
类别
常规抗体
推荐应用
WB
反应种属
Human
Human Swissprot No.
Q92954
特异性
This PRG4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 176-210 amino acids from the human region of human PRG4.
稀释度
IF~~1:25;WB~~1:500
预测分子量
151kDa
同种型
Rabbit Ig
背景介绍
Plays a role in boundary lubrication within articulating joints. Prevents protein deposition onto cartilage from synovial fluid by controlling adhesion-dependent synovial growth and inhibiting the adhesion of synovial cells to the cartilage surface.
组织表达
Highly expressed in synovial tissue, cartilage and liver and weakly in heart and lung. Isoform B is expressed in kidney, lung, liver, heart and brain. Isoform C and isoform D are widely expressed.
细胞定位
Secreted.
期货
现货

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hela (human cervical epithelial adenocarcinoma cell line) cells labeling PRG4 with BD-PB3748 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on Hela cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).

Anti-PRG4 Antibody (N-Term) at 1:500 dilution + Human liver whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 151 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

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