BD-PB3747-MUT Rabbit Polyclonal Antibody (Center)-现货抗体产品库武汉枢密脑科学技术有限公司

MUT Rabbit Polyclonal Antibody (Center)

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产品基本信息

产品货号

BD-PB3747

产品名称

MUT Rabbit Polyclonal Antibody (Center)

别名

Methylmalonyl-CoA mutase, mitochondrial, MCM, 5.4.99.2, Methylmalonyl-CoA isomerase, MUT

类别

常规抗体

推荐应用

反应种属

Human, Mouse

Human Swissprot No.

P22033

特异性

This MUT antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 492-526 amino acids from the Central region of human MUT.

稀释度

IF~~1:25;WB~~1:2000

预测分子量

83kDa

同种型

Rabbit Ig

背景介绍

Involved in the degradation of several amino acids, odd- chain fatty acids and cholesterol via propionyl-CoA to the tricarboxylic acid cycle. MCM has different functions in other species.

细胞定位

Mitochondrion matrix. Mitochondrion. Cytoplasm

期货

现货

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hela (human cervical epithelial adenocarcinoma cell line) cells labeling MUT with BD-PB3747 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm mainly and mitochondrion staining on Hela cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).

All lanes : Anti-MUT Antibody (Center) at 1:2000 dilution Lane 1: Human liver lysate Lane 2: Mouse liver lysate Lane 3: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : kDa Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes : Anti-MUT Antibody (Center) at 1:2000 dilution Lane 1: K562 whole cell lysate Lane 2: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 83 kDa Blocking/Dilution buffer: 5% NFDM/TBST.