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COXIV Rabbit Polyclonal Antibody

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产品基本信息

产品货号
BD-PB3744
产品名称
COXIV Rabbit Polyclonal Antibody
别名
Cytochrome c oxidase subunit 4 isoform 1, mitochondrial, Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit IV isoform 1, COX IV-1, Cox4i1, Cox4, Cox4a
类别
常规抗体
推荐应用
WB
反应种属
Human, Rat, Mouse
Human Swissprot No.
P19783
特异性
This COXIV antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 49-83 amino acids from the mouse region of mouse COXIV.
稀释度
IF~~1:25;WB~~1:2000
预测分子量
20kDa
同种型
Rabbit Ig
背景介绍
This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
细胞定位
Mitochondrion inner membrane {ECO:0000250|UniProtKB:P00423}; Single-pass membrane protein {ECO:0000250|UniProtKB:P00423}
期货
现货

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized NIH/3T3 cells labeling Cox4i1 with BD-PB3744 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-Rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing Cytoplasm staining on NIH/3T3 cell line. The nuclear counter stain is DAPI (blue).

Anti-COXIV Antibody at 1:2000 dilution + BA/F3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 17 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

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