产品名称
BOREA Rabbit Polyclonal Antibody
蛋白名称
Borealin (Cell division cycle-associated protein 8) (Dasra-B) (hDasra-B) (Pluripotent embryonic stem cell-related gene 3 protein)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
Q53HL2
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q53HL2/entry
Mouse Swissprot No.
Q8BHX3
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q8BHX3
Rat Swissprot Link
http://www.uniprot.org/uniprot/O54941Q6AXW0
免疫原
Synthesized peptide derived from part region of human protein
特异性
BOREA Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
cell division cycle associated 8(CDCA8) Homo sapiens This gene encodes a component of the chromosomal passenger complex. This complex is an essential regulator of mitosis and cell division. This protein is cell-cycle regulated and is required for chromatin-induced microtubule stabilization and spindle formation. Alternate splicing results in multiple transcript variants. Pseudgenes of this gene are found on chromosomes 7, 8 and 16. [provided by RefSeq, Apr 2013],
组织表达
Colon,Coronary arterial endothelium,Embryo,Epithelium,Kidney,Lung,
细胞定位
Nucleus, nucleolus . Cytoplasm . Cytoplasm, cytoskeleton, spindle . Chromosome, centromere . Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with SENP3 in the nucleolus in interphase cells. .
功能
developmental stage:Cell-cycle regulated. Increases during G2/M phase and then reduces after exit from M phase.,function:Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA.,miscellaneous:Cells lacking CDCA8 display a slight decrease in histone H3 'Ser-10' phosphorylation, suggesting that the CPC complex mediates phosphorylation of 'Ser-10' of histone H3.,similarity:Belongs to the borealin family.,subcellular location:Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis.,subunit:May form homooligomers. Component of the CPC at least composed of BIRC5/survivin, CDCA8/borealin, INCENP and AURKB/Aurora-B.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
