BD-PB3715-BIRC6 Rabbit Polyclonal Antibody (C-Term)-现货抗体产品库武汉枢密脑科学技术有限公司

BIRC6 Rabbit Polyclonal Antibody (C-Term)

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产品基本信息

产品货号

BD-PB3715

产品名称

BIRC6 Rabbit Polyclonal Antibody (C-Term)

别名

Baculoviral IAP repeat-containing protein 6, 6.3.2.-, BIR repeat-containing ubiquitin-conjugating enzyme, BRUCE, Ubiquitin-conjugating BIR domain enzyme apollon, APOLLON, BIRC6, KIAA1289

类别

常规抗体

推荐应用

反应种属

Human, Mouse

Human Swissprot No.

Q9NR09

特异性

This BIRC6 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 4810-4844 amino acids from human BIRC6.

稀释度

FC~~1:25;WB~~1:1000

预测分子量

530kDa

同种型

Rabbit Ig

背景介绍

Anti-apoptotic protein which can regulate cell death by controlling caspases and by acting as an E3 ubiquitin-protein ligase. Has an unusual ubiquitin conjugation system in that it could combine in a single polypeptide, ubiquitin conjugating (E2) with ubiquitin ligase (E3) activity, forming a chimeric E2/E3 ubiquitin ligase. Its tragets include CASP9 and DIABLO/SMAC. Acts as an inhibitor of CASP3, CASP7 and CASP9. Important regulator for the final stages of cytokinesis. Crucial for normal vesicle targeting to the site of abscission, but also for the integrity of the midbody and the midbody ring, and its striking ubiquitin modification.

组织表达

Expressed in brain cancer cells.

细胞定位

Golgi apparatus, trans-Golgi network membrane. Endosome Cytoplasm, cytoskeleton, spindle pole Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Midbody, Midbody ring. Note=Exhibits cell cycle-dependent localization. Concentrates in a pericentriolar compartment in interphase, moves partially to spindle poles in metaphase, and finally localizes to the spindle midzone and the midbody in telophase and during cytokinesis. On the midbody, localizes to the midbody ring, also called Flemming body (PubMed:18329369). In interphase cells, localizes to the trans-Golgi network membrane and endosomes. During cytokinesis, a fraction moves to the midzone where it specifically arrives at the midbody ring. After abscission completion, travels with the midbody remnant into one daughter cell, and remains bound to it until a new midbody ring is formed during the next cell division (PubMed:18329369)

期货

现货

Overlay histogram showing Hela cells stained with BD-PB3715(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

All lanes : Anti-BIRC6 Antibody (C-Term) at 1:1000 dilution Lane 1: SH-SY5Y whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 530 kDa Blocking/Dilution buffer: 5% NFDM/TBST.