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HIF1AN (7A17)Mouse Monoclonal Antibody(C-term)

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产品基本信息

产品货号
BD-PB3707
产品名称
HIF1AN (7A17)Mouse Monoclonal Antibody(C-term)
别名
Hypoxia-inducible factor 1-alpha inhibitor, 1.14.11.30, 1.14.11.n4, Factor inhibiting HIF-1, FIH-1, Hypoxia-inducible factor asparagine hydroxylase, HIF1AN, FIH1
类别
常规抗体
推荐应用
WB
反应种属
Human
Human Swissprot No.
Q9NWT6
特异性
This HIF1AN antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 1-349 amino acids from human HIF1AN.
稀释度
IF~~1:25;WB~~1:4000
预测分子量
40kDa
同种型
IgG1,k
背景介绍
Hydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300- interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation.
细胞定位
Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Note=Mainly cytoplasmic localization, but interaction with NOTCH1 results in nuclear localization and interaction with ABPA3 results in perinuclear localization in macrophages
期货
现货

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling HIF1AN with BD-PB3707 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing mitochondrion staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).

All lanes : Anti-HIF1AN Antibody (C-term) at 1:4000 dilution Lane 1: 293 whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: ZR-75-1 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

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