BD-PB3683-KANK1(7V1)Mouse Monoclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

KANK1(7V1)Mouse Monoclonal Antibody

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产品基本信息

产品货号

BD-PB3683

产品名称

KANK1(7V1)Mouse Monoclonal Antibody

别名

KN motif and ankyrin repeat domain-containing protein 1, Ankyrin repeat domain-containing protein 15, Kidney ankyrin repeat-containing protein, KANK1, ANKRD15, KANK, KIAA0172

类别

常规抗体

推荐应用

反应种属

Human

Human Swissprot No.

Q14678

特异性

This KANK1 antibody is generated from a mouse immunized with a recombinant protein of human KANK1.

稀释度

IHC-P~~1:100~500;WB~~1:2000

预测分子量

147kDa

同种型

IgG1,k

背景介绍

Involved in the control of cytoskeleton formation by regulating actin polymerization. Inhibits actin fiber formation and cell migration. Inhibits RhoA activity; the function involves phosphorylation through PI3K/Akt signaling and may depend on the competetive interaction with 14-3-3 adapter proteins to sequester them from active complexes. Inhibits the formation of lamellipodia but not of filopodia; the function may depend on the competetive interaction with BAIAP2 to block its association with activated RAC1. Inhibits fibronectin-mediated cell spreading; the function is partially mediated by BAIAP2. Inhibits neurite outgrowth. Involved in the establishment and persistence of cell polarity during directed cell movement in wound healing. In the nucleus, is involved in beta-catenin-dependent activation of transcription. Potential tumor suppressor for renal cell carcinoma.

组织表达

Widely expressed. Isoform 1 is predominantly expressed in heart and kidney. Isoform 2 probably is widely expressed at basic levels.

细胞定位

Cell projection, ruffle membrane. Cytoplasm. Nucleus Note=Colocalizes with KIF21A in membrane ruffles (PubMed:19559006) Shuttles between the cytoplasm and nucleus (PubMed:16968744) [Isoform 2]: Cytoplasm. Nucleus Note=Shuttles between the cytoplasm and nucleus

期货

现货

BD-PB3683 staining KANK1 in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

All lanes : Anti-KANK1 Antibody at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 147 kDa Blocking/Dilution buffer: 5% NFDM/TBST.