All lanes : Anti-GMPPB Antibody (N-Term) at 1:2000 dilution Lane 1: human pancreas lysate Lane 2: mouse pancreas lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST.