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GARS(5D14)Mouse Monoclonal Antibody

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产品基本信息

产品货号
BD-PB3567
产品名称
GARS(5D14)Mouse Monoclonal Antibody
别名
Glycine--tRNA ligase, 6.1.1.14, Diadenosine tetraphosphate synthetase, AP-4-A synthetase, Glycyl-tRNA synthetase, GlyRS, GARS
类别
常规抗体
推荐应用
WB
反应种属
Human, Rat, Mouse
Human Swissprot No.
P41250
特异性
This GARS antibody is generated from a mouse immunized with a recombinant protein between 15-305 amino acids from human GARS.
稀释度
IF~~1:25;WB~~1:4000
预测分子量
83kDa
同种型
IgG1,k
背景介绍
Catalyzes the attachment of glycine to tRNA(Gly). Is also able produce diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs.
组织表达
Widely expressed, including in brain and spinal cord. [Isoform 1]: Expressed in brain, spinal cord, muscle, heart, spleen and liver.
细胞定位
Cytoplasm. Cell projection, axon. Secreted {ECO:0000250|UniProtKB:Q9CZD3}. Secreted, extracellular exosome {ECO:0000250|UniProtKB:Q9CZD3}. Note=In transfected COS7 cells, not detected in mitochondria, nor in Golgi apparatus (PubMed:17035524) Secreted by motor neuron, possibly through the exosome pathway (By similarity). {ECO:0000250|UniProtKB:Q9CZD3, ECO:0000269|PubMed:17035524} [Isoform 2]: Cytoplasm. Cell projection, axon
期货
现货

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling GARS with BD-PB3567 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and weak nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).

All lanes : Anti-GARS Antibody at 1:4000 dilution Lane 1: HT-1080 whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: Ramos whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 83 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

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