BD-PB3566-BANP(8J11)Mouse Monoclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

BANP(8J11)Mouse Monoclonal Antibody

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产品基本信息

产品货号

BD-PB3566

产品名称

BANP(8J11)Mouse Monoclonal Antibody

别名

Protein BANP, BEN domain-containing protein 1, Btg3-associated nuclear protein, Scaffold/matrix-associated region-1-binding protein, BANP, BEND1, SMAR1

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

Human Swissprot No.

Q8N9N5

特异性

This BANP antibody is generated from a mouse immunized with a recombinant protein between 30-390 amino acids from human BANP.

稀释度

IHC-P~~1:100~500;WB~~1:1000

预测分子量

56kDa

同种型

IgG1,κ

背景介绍

Controls V(D)J recombination during T-cell development by repressing T-cell receptor (TCR) beta enhancer function. Binds to scaffold/matrix attachment region beta (S/MARbeta), an ATC-rich DNA sequence located upstream of the TCR beta enhancer. Represses cyclin D1 transcription by recruiting HDAC1 to its promoter, thereby diminishing H3K9ac, H3S10ph and H4K8ac levels. Promotes TP53 'Ser-15' phosphorylation and nuclear accumulation, which causes cell cycle arrest (By similarity).

组织表达

Down-regulated in breast cancer cell lines.

细胞定位

Nucleus.

期货

现货

BD-PB3566 staining BANP in human breast carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Anti-BANP Antibody at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 56 kDa Blocking/Dilution buffer: 5% NFDM/TBST.