All lanes : Anti-IDNK Antibody (N-Term) at 1:8000 dilution Lane 1: NCI-H460 whole cell lysate Lane 2: DU145 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 21 kDa Blocking/Dilution buffer: 5% NFDM/TBST.