Anti-ACTB Antibody, HRP Conjugate at 1:2000 dilution Lane 1: NIH/3T3 whole cell lysate Lane 2: Mouse brain lysate Lane 3: COLO 205 whole cell lysate Lane 4: Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Observed band size: 42 KDa Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-ACTB Antibody, HRP Conjugate at 1:2000 dilution + Hela whole cell lysate Lysates/proteins at 20 µg per lane. Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.