BD-PB3477-RAB11A(5P3)Mouse Monoclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

RAB11A(5P3)Mouse Monoclonal Antibody

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产品基本信息

产品货号

BD-PB3477

产品名称

RAB11A(5P3)Mouse Monoclonal Antibody

别名

Ras-related protein Rab-11A, Rab-11, YL8, RAB11A, RAB11

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

Human Swissprot No.

P62491

特异性

This RAB11A antibody is generated from a mouse immunized with a purified recombinant protein of human RAB11A.

稀释度

WB~~1:4000;IHC-P~~1:100~500

预测分子量

24kDa

同种型

IgG1,κ

背景介绍

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab regulates endocytic recycling. Acts as a major regulator of membrane delivery during cytokinesis. Together with MYO5B and RAB8A participates in epithelial cell polarization. Together with RAB3IP, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B participates in CFTR trafficking to the plasma membrane and TF (Transferrin) recycling in nonpolarized cells. Required in a complex with MYO5B and RAB11FIP2 for the transport of NPC1L1 to the plasma membrane. Participates in the sorting and basolateral transport of CDH1 from the Golgi apparatus to the plasma membrane. Regulates the recycling of FCGRT (receptor of Fc region of monomeric Ig G) to basolateral membranes. May also play a role in melanosome transport and release from melanocytes.

细胞定位

Cell membrane; Lipid-anchor. Recycling endosome membrane; Lipid-anchor. Cleavage furrow. Cytoplasmic vesicle, phagosome. Cytoplasmic vesicle membrane. Note=Translocates with RAB11FIP2 from the vesicles of the endocytic recycling compartment (ERC) to the plasma membrane (PubMed:11994279). Localizes to the cleavage furrow (PubMed:15601896). Colocalizes with PARD3, PRKCI, EXOC5, OCLN, PODXL and RAB8A in apical membrane initiation sites (AMIS) during the generation of apical surface and lumenogenesis (PubMed:20890297) Recruited to phagosomes containing S.aureus or M.tuberculosis (PubMed:21255211).

期货

现货

All lanes : Anti-RAB11A Antibody at 1:4000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: C2C12 whole cell lysate Lane 4: PC-12 whole cell lysate Lane 5: rat brain lysate Lane 6: 293T/17 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 24 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

BD-PB3477 staining RAB11A in human brain sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.