All lanes : Anti-OR2T7 Antibody (C-term) at 1:2000 dilution Lane 1: human brain lysate Lane 2: HT-29 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 34 kDa Blocking/Dilution buffer: 5% NFDM/TBST.