BD-PB3388-PSMB7 Rabbit Polyclonal Antibody (Center)-现货抗体产品库武汉枢密脑科学技术有限公司

PSMB7 Rabbit Polyclonal Antibody (Center)

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产品基本信息

产品货号

BD-PB3388

产品名称

PSMB7 Rabbit Polyclonal Antibody (Center)

别名

Proteasome subunit beta type-7, Macropain chain Z, Multicatalytic endopeptidase complex chain Z, Proteasome subunit Z, PSMB7, Z

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

Human Swissprot No.

Q99436

特异性

This PSMB7 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 180-211 amino acids from the Central region of human PSMB7.

稀释度

IHC-P~~1:100~500;WB~~1:8000

预测分子量

30kDa

同种型

Rabbit Ig

背景介绍

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the trypsin-like activity.

组织表达

Expressed at a low level in colonic mucosa. Up- regulated in colorectal cancer tissues.

细胞定位

Cytoplasm. Nucleus

期货

现货

BD-PB3388 staining PSMB7 in human colorectal carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

All lanes : Anti-PSMB7 Antibody (Center) at 1:2000 dilution Lane 1: Raji whole cell lysates Lane 2: Hela whole cell lysates Lane 3: NIH/3T3 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 30 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes : Anti-PSMB7 Antibody (Center) at 1:8000 dilution Lane 1: Jurkat whole cell lysates Lane 2: A375 whole cell lysates Lane 3: HepG2 whole cell lysates Lane 4: MCF-7 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 30 kDa Blocking/Dilution buffer: 5% NFDM/TBST.