BD-PB3386-RNF7 Rabbit Polyclonal Antibody (N-term)-现货抗体产品库武汉枢密脑科学技术有限公司

RNF7 Rabbit Polyclonal Antibody (N-term)

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产品基本信息

产品货号

BD-PB3386

产品名称

RNF7 Rabbit Polyclonal Antibody (N-term)

别名

RING-box protein 2, Rbx2, CKII beta-binding protein 1, CKBBP1, RING finger protein 7, Regulator of cullins 2, Sensitive to apoptosis gene protein, RNF7, RBX2, ROC2, SAG

类别

常规抗体

推荐应用

反应种属

Human, Mouse

Human Swissprot No.

Q9UBF6

特异性

This RNF7 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 25-57 amino acids from the N-terminal region of human RNF7.

稀释度

IHC-P~~1:100~500;WB~~1:8000

预测分子量

13kDa

同种型

Rabbit Ig

背景介绍

Probable component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme to the complex and brings it into close proximity to the substrate. Promotes the neddylation of CUL5 via its interaction with UBE2F. May play a role in protecting cells from apoptosis induced by redox agents.

组织表达

Expressed in heart, liver, skeletal muscle and pancreas. At very low levels expressed in brain, placenta and lung

细胞定位

Cytoplasm. Nucleus

期货

现货

BD-PB3386 staining RNF7 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

All lanes : Anti-RNF7 Antibody (N-term) at 1:8000 dilution Lane 1: human heart lysates Lane 2: human liver lysates Lane 3: human skeletal muscle lysates Lane 4: human testis lysates Lane 5: mouse heart lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 13 kDa Blocking/Dilution buffer: 5% NFDM/TBST.