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BCL9 Rabbit Polyclonal Antibody (N-Term)

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产品基本信息

产品货号
BD-PB3358
产品名称
BCL9 Rabbit Polyclonal Antibody (N-Term)
别名
B-cell CLL/lymphoma 9 protein, B-cell lymphoma 9 protein, Bcl-9, Protein legless homolog, BCL9
类别
常规抗体
推荐应用
WB
反应种属
Human, Mouse
Human Swissprot No.
O00512
特异性
This BCL9 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 272-304 amino acids from human BCL9.
稀释度
IF~~1:25;WB~~1:2000
预测分子量
149kDa
同种型
Rabbit Ig
背景介绍
Involved in signal transduction through the Wnt pathway. Promotes beta-catenin's transcriptional activity (By similarity).
组织表达
Detected at low levels in thymus, prostate, testis, ovary and small intestine, and at lower levels in spleen, colon and blood
细胞定位
Nucleus.
期货
现货

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling BCL9 with BD-PB3358 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).

All lanes : Anti-BCL9 Antibody (N-Term) at 1:2000 dilution Lane 1: mouse spleen lysates Lane 2: human testis lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 149 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

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