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AKR7L Rabbit Polyclonal Antibody (N-Term)

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产品基本信息

产品货号
BD-PB3352
产品名称
AKR7L Rabbit Polyclonal Antibody (N-Term)
别名
Aflatoxin B1 aldehyde reductase member 4, 1---, AFB1 aldehyde reductase 3, AFB1-AR 3, Aldoketoreductase 7-like, AKR7L, AFAR3, AKR7A4
类别
常规抗体
推荐应用
WB
反应种属
Human, Rat, Mouse
Human Swissprot No.
Q8NHP1
特异性
This AKR7L antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 32-64 amino acids from human AKR7L.
稀释度
IF~~1:25;WB~~1:2000
预测分子量
37kDa
同种型
Rabbit Ig
背景介绍
Can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the non-binding AFB1 dialcohol. May be involved in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen (By similarity).
组织表达
Mainly expressed in uterus.
期货
现货

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling AKR7L with BD-PB3352 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HepG2 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).

Anti-AKR7L Antibody (N-Term)at 1:2000 dilution + HepG2 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

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