BD-PB3317-Mouse Med12 Rabbit Polyclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

Mouse Med12 Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号

BD-PB3317

产品名称

Mouse Med12 Rabbit Polyclonal Antibody (C-term)

别名

Mediator of RNA polymerase II transcription subunit 12, Mediator complex subunit 12, OPA-containing protein, Thyroid hormone receptor-associated protein complex 230 kDa component, Trap230, Trinucleotide repeat-containing gene 11 protein, Med12, Kiaa0192, Mopa, Tnrc11, Trap230

类别

常规抗体

推荐应用

反应种属

Human, Mouse

Human Swissprot No.

A2AGH6

特异性

This Mouse Med12 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1991-2025 amino acids from the C-terminal region of Mouse Med12.

稀释度

WB~~1:2000;IHC-P~~1:100~500

预测分子量

245kDa

同种型

Rabbit Ig

背景介绍

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway (By similarity).

细胞定位

Nucleus.

期货

现货

Anti-Med12 Antibody (C-term)at 1:1000 dilution + mouse spleen lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 245 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

BD-PB3317 staining Mouse Med12 in mouse stomach sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Anti-Med12 Antibody (C-term)at 1:1000 dilution + NIH/3T3 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 245 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes : Anti-Med12 Antibody (C-term) at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: C2C12 whole cell lysates Lane 3: Jurkat whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 245 kDa Blocking/Dilution buffer: 5% NFDM/TBST.