产品名称
(Mouse) Smad3 Rabbit Polyclonal Antibody (Center)
别名
Mothers against decapentaplegic homolog 3, MAD homolog 3, Mad3, Mothers against DPP homolog 3, mMad3, SMAD family member 3, SMAD 3, Smad3, Smad3, Madh3
Human Swissprot No.
Q8BUN5
特异性
This Mouse Smad3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 157-191 amino acids from the Central region of Mouse Smad3.
背景介绍
Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF- mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator (By similarity).
组织表达
Highly expressed in the brain and ovary. Detected in the pyramidal cells of the hippocampus, granule cells of the dentate gyrus, granular cells of the cerebral cortex and the granulosa cells of the ovary.
细胞定位
Cytoplasm. Nucleus. Note=Cytoplasmic and nuclear in the absence of TGF-beta (PubMed:21145499). On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4 (PubMed:21145499) Through the action of the phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1 (By similarity). Co-localizes with LEMD3 at the nucleus inner membrane (By similarity). MAPK-mediated phosphorylation appears to have no effect on nuclear import. PDPK1 prevents its nuclear translocation in response to TGF-beta (By similarity). Localized mainly to the nucleus in the early stages of embryo development with expression becoming evident in the cytoplasm of the inner cell mass at the blastocyst stage (PubMed:21145499). {ECO:0000250|UniProtKB:P84022, ECO:0000269|PubMed:21145499}
